Applications

Selected Application Examples

Quantitative comparison study of flow cytometers using a novel ultra-stable calibration light source quantiflash

26th Annual Meeting of the German Society for Cytometry, Oral Presentation

Konrad von Volkmann, Oral Presentation: Quantitative comparison study of flow cytometers using a novel ultra-stable calibration light source

In our talk we will discuss ways quantify flow cytometers in terms of sensitivity and resolution. As shown by others, the coefficient of variation (CV) of a stable light source can be used for scale calibration in numbers of detected photoelectrons. This calibration allows the quantitative comparison of flow cytometers in terms of light detection efficiency. We will present a comparison study of various cytometers available in the Core Facility of the DRFZ employing a novel calibration light source, called quantiFlash.

The full presenation is available for download HERE.

 

 

Forensic Flow Cytometry Calibration using APEs quantiFlash

Forensic Flow Cytometry

Pratip Chattopadhyay, Staff Scientist at National Institutes of Health, presented a Forensic Flow Cytometry Tutorial at the CYTO2015 Conference.

The full presenation is available at http://de.slideshare.net/PratipChattopadhyay/cyto-2015-forensic-flow-tutorial

 

 

DRfZ

Comparison study of various flow cytometers using quantiFlash. Traditionally, flow cytometers are characterized (sensitivity, linearity, long time stability etc.) by fluorescent microspheres.

Comparison study of various flow cytometers using quantiFlash. Traditionally, flow cytometers are characterized (sensitivity, linearity, long time stability etc.) by fluorescent microspheres. As shown by others*) the coefficient of variation (CV) of a stable light source can be used for scale calibration in numbers of estimated photoelectrons. This allows the quantitative comparison of flow cytometers in terms of light detection efficiency. Due to the intrinsic CV (2-4%) of microspheres they are not suitable for such calibration. Here we show a comparison of the detection efficiency of 3 flow cytometers (FACSAria™) using quantiFlash™. quantiFlash™ is an ultra-stable (CV < 0.1%) easy to use LED pulse generator made for cytometer characterization.

The poster has been presented at CYTO 2016, Seattle.

This poster is available for download.

 

Characterization of Extracellular Vesicles

Calibration of flow cytometric analysis and characterization of background noise sources that may impact EV fluorescence or scatter detection.

Transkript von Isolation and characterization of Extracellular Vesicles

Flow cytometric detection and sorting of EVs: analysis and characterization of background noise sources that may impact EV fluorescence or scatter detection
by Dr. Carley D. Ross, Dr. Thomas Ramin, Dr. Aliaksandr Kachynski
Beckman Coulter Life Sciences
Cellular and Molecular Life Sciences 2011; 68(16):2667-88.

The full presentation is available at https://prezi.com/loogalklhahr/isolation-and-characterization-of-extracellular-vesicles/